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end htslp  (R&D Systems)


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    Structured Review

    R&D Systems end htslp
    End Htslp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+tslp/Recombinant+Human+TSLP+Protein/us12492248-820-2-13
    Average 93 stars, based on 41 article reviews
    end htslp - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: Nucleic acids encoding polypeptides comprising immunoglobulin single variable domains targeting IL-13 and TSLP
    Article Snippet: CCL17 production in freshly collected cell culture supernatant was measured by ELISA and IC50 values of the ISVD construct and benchmark antibody were calculated in Graphpad Prism.

    Article Title: Nucleic acids encoding polypeptides comprising immunoglobulin single variable domains targeting IL-13 and TSLP
    Article Snippet: After blocking and adding diluted serum samples, the presence of anti-IL-13 and anti-TSLP ISVDs was demonstrated by using HRP (horseradish peroxidase) conjugated goat anti-llama immunoglobulin (Bethyl Laboratories Inc.) and a subsequent enzymatic reaction in the presence of the substrate TMB (3,3′,5,5′-tetramentylbenzidine).

    Recombinant:

    Article Title: Methods of treatment utilizing polypeptides comprising immunoglobulin single variable domains targeting IL-13 and TSLP
    Article Snippet: .. Recombinant human TSLP is commercially available, such as from R&D Systems (Catalog number 1398-TS). .. CCL17 production in freshly collected cell culture supernatant was measured by ELISA and IC50 values of the ISVD construct and benchmark antibody were calculated in Graphpad Prism.

    Article Title: Agents that interfere with thymic stromal lymphopoietin (TSLP)-receptor signaling
    Article Snippet: .. In this assay, 1 μg/mL recombinant human TSLP (R&D Systems) were coated on ELISA plate. ..

    Article Title: Thymic stromal lymphopoietin contributes to endometriotic lesion proliferation and disease-associated inflammation
    Article Snippet: .. After 24 h of rest, cells were treated in triplicates with vehicle control (PBS; 10% v/v) or recombinant human TSLP (rhTSLP; 1398-TS-010/CF, R&D Systems, USA) (1, 5, 10, 50, 100 ng/mL) for 24 h. To establish whether TSLP treatment impacts the cell lines’ proliferation, wells were treated with 10 μL of WST-I reagent (501594401, Millipore Sigma, Canada) for 2 h at 37°C with 5% CO 2 . .. Absorbance, proportional to the amount of metabolically-active cells in culture, was quantified with the SpectraMaxiD3 microplate reader (Molecular Devices, USA) at a wavelength reading of 440 nm.

    Article Title: Methods of treatment utilizing polypeptides comprising immunoglobulin single variable domains targeting IL-13 and TSLP
    Article Snippet: Briefly, 96-well Maxisorp plates were coated with human IL-13 (Sino Biological, cat nr 10369-HNAC) or human TSLP. .. Recombinant human TSLP is commercially available, such as from R&D Systems (cat nr 1398-TS). .. After blocking and adding diluted serum samples, the presence of anti-IL-13 and anti-TSLP ISVDs was demonstrated by using HRP (horseradish peroxidase) conjugated goat anti-llama immunoglobulin (Bethyl Laboratories Inc.) and a subsequent enzymatic reaction in the presence of the substrate TMB (3,3′,5,5′-tetramentylbenzidine).

    Article Title: Methods of treating asthma with polypeptides targeting IL-13 and TSLP
    Article Snippet: .. Recombinant human TSLP is commercially available, such as from R&D Systems (Catalog number 1398-TS). .. CCL17 production in freshly collected cell culture supernatant was measured by ELISA and IC50 values of the ISVD construct and benchmark antibody were calculated in Graphpad Prism.

    Article Title: A Bispecific Antibody Blocking Both TSLP and IL-4Rα for the Treatment of Allergic Inflammatory Diseases
    Article Snippet: HEK293T cells were co-transfected with constructs expressing IL-7Rα, TSLPR, and pGL4.52 STAT5 luciferase reporter gene (Promega, Madison, MI, USA) by lipofectamine 3000 (ThermoFisher Scientific, San Jose, CA, USA). .. One day post-transfection, testing antibodies along with 3 ng/mL recombinant human TSLP (R&D systems, Minneapolis, MN, USA) were applied to the transfected cells and incubated for 24 h. The luciferase reporter gene activity was determined using the ONE-GloTM EX Luciferase Assay kit (Promega, Madison, MI, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Agents that interfere with thymic stromal lymphopoietin (TSLP)-receptor signaling
    Article Snippet: .. In this assay, 1 μg/mL recombinant human TSLP (R&D Systems) were coated on ELISA plate. ..

    Control:

    Article Title: Thymic stromal lymphopoietin contributes to endometriotic lesion proliferation and disease-associated inflammation
    Article Snippet: .. After 24 h of rest, cells were treated in triplicates with vehicle control (PBS; 10% v/v) or recombinant human TSLP (rhTSLP; 1398-TS-010/CF, R&D Systems, USA) (1, 5, 10, 50, 100 ng/mL) for 24 h. To establish whether TSLP treatment impacts the cell lines’ proliferation, wells were treated with 10 μL of WST-I reagent (501594401, Millipore Sigma, Canada) for 2 h at 37°C with 5% CO 2 . .. Absorbance, proportional to the amount of metabolically-active cells in culture, was quantified with the SpectraMaxiD3 microplate reader (Molecular Devices, USA) at a wavelength reading of 440 nm.

    Transfection:

    Article Title: A Bispecific Antibody Blocking Both TSLP and IL-4Rα for the Treatment of Allergic Inflammatory Diseases
    Article Snippet: HEK293T cells were co-transfected with constructs expressing IL-7Rα, TSLPR, and pGL4.52 STAT5 luciferase reporter gene (Promega, Madison, MI, USA) by lipofectamine 3000 (ThermoFisher Scientific, San Jose, CA, USA). .. One day post-transfection, testing antibodies along with 3 ng/mL recombinant human TSLP (R&D systems, Minneapolis, MN, USA) were applied to the transfected cells and incubated for 24 h. The luciferase reporter gene activity was determined using the ONE-GloTM EX Luciferase Assay kit (Promega, Madison, MI, USA). ..

    Incubation:

    Article Title: A Bispecific Antibody Blocking Both TSLP and IL-4Rα for the Treatment of Allergic Inflammatory Diseases
    Article Snippet: HEK293T cells were co-transfected with constructs expressing IL-7Rα, TSLPR, and pGL4.52 STAT5 luciferase reporter gene (Promega, Madison, MI, USA) by lipofectamine 3000 (ThermoFisher Scientific, San Jose, CA, USA). .. One day post-transfection, testing antibodies along with 3 ng/mL recombinant human TSLP (R&D systems, Minneapolis, MN, USA) were applied to the transfected cells and incubated for 24 h. The luciferase reporter gene activity was determined using the ONE-GloTM EX Luciferase Assay kit (Promega, Madison, MI, USA). ..

    Luciferase:

    Article Title: A Bispecific Antibody Blocking Both TSLP and IL-4Rα for the Treatment of Allergic Inflammatory Diseases
    Article Snippet: HEK293T cells were co-transfected with constructs expressing IL-7Rα, TSLPR, and pGL4.52 STAT5 luciferase reporter gene (Promega, Madison, MI, USA) by lipofectamine 3000 (ThermoFisher Scientific, San Jose, CA, USA). .. One day post-transfection, testing antibodies along with 3 ng/mL recombinant human TSLP (R&D systems, Minneapolis, MN, USA) were applied to the transfected cells and incubated for 24 h. The luciferase reporter gene activity was determined using the ONE-GloTM EX Luciferase Assay kit (Promega, Madison, MI, USA). ..

    Activity Assay:

    Article Title: A Bispecific Antibody Blocking Both TSLP and IL-4Rα for the Treatment of Allergic Inflammatory Diseases
    Article Snippet: HEK293T cells were co-transfected with constructs expressing IL-7Rα, TSLPR, and pGL4.52 STAT5 luciferase reporter gene (Promega, Madison, MI, USA) by lipofectamine 3000 (ThermoFisher Scientific, San Jose, CA, USA). .. One day post-transfection, testing antibodies along with 3 ng/mL recombinant human TSLP (R&D systems, Minneapolis, MN, USA) were applied to the transfected cells and incubated for 24 h. The luciferase reporter gene activity was determined using the ONE-GloTM EX Luciferase Assay kit (Promega, Madison, MI, USA). ..



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